Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
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Plant Physiology 72:398-401 (1983)
© 1983 American Society of Plant Biologists

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Enzyme-Linked Immunosorbent Assay of Fungal NADP+-Glutamate Dehydrogenase 1

Francis Martin2, Bernard Botton and Yamina Msatef

Laboratoire de Microbiologie et Physiologie Forestières, Centre de Recherches Forestières, Institut National de la Recherche Agronomique, Champenoux 54280 Seichamps, France, Laboratoire de Physiologie Végétale, Faculté des Sciences, Université de Nancy 1—B.P. 239—54506 Vandoeuvre-les-Nancy Cedex, France

A sensitive and reliable method has been developed for the quantitation of NADP+-glutamate dehydrogenase from the phytopathogenic Ascomycete Sphaerostilbe repens using a two-step competitive enzyme-linked immunosorbent assay. Purified enzyme was adsorbed noncovalently to polystyrene wells and rabbit immunserum was allowed to bind to antigensensitized wells. Bound specific antibody was visualized by goat antirabbit immunoglobulin covalently linked to alkaline phosphatase using paranitrophenylphosphate as the substrate. Increasing amounts of purified enzyme or crude fungal extracts were quantitated by their ability to inhibit specific antibody adsorption to antigen-coated polystyrene wells. This system proves to be useful in the range of 10 to 80 nanograms of enzyme level. Using this assay, identical amounts of NADP+-glutamate dehydrogenase were found in mycelia grown on nitrate and ammonia sources.

2 To whom correspondence should be addressed.

1 Supported by a fellowship from the Moroccan Government (Y. M.).






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Copyright © 1983 by the American Society of Plant Biologists