This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Web of Science (6) Citing Articles via Google Scholar Google Scholar Articles by Ishii, S. Articles by Mogi, Y. Search for Related Content PubMed PubMed Citation Articles by Ishii, S. Articles by Mogi, Y. Agricola Articles by Ishii, S. Articles by Mogi, Y.

Articles

Identification of Enzymes That Are Effective for Isolating Protoplasts from Grass Leaves

Central Research Laboratories, Kikkoman Corporation, 399 Noda, Noda-shi, Chiba-ken 278 Japan

Cellulase C₁, cellulase Cx, and xylanase were isolated and purified from a cellulase preparation of Trichoderma viride as enzymes effective in the isolation of protoplasts from oat leaves. Pectin lyase which is specific for methyl-galacturonide linkages was also found to be a useful enzyme for the isolation of protoplasts from the tissues. This suggested that pectic polysaccharides with a high degree of esterification may play an important role in cell walls of Gramineae. It was necessary to use the mixture of cellulase C₁, cellulase Cx, xylanase, and pectin lyase for the rapid isolation of protoplasts, while a small amount of protoplasts could be isolated from oat leaves by cellulase C₁ plus xylanase or cellulase C₁ plus pectin lyase. The mixture of four enzymes also was effective in the isolation of protoplasts from the leaves of wheat, barley, and corn.