This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Stiekema, W. J. Articles by Tobin, E. M. Search for Related Content PubMed PubMed Citation Articles by Stiekema, W. J. Articles by Tobin, E. M. Agricola Articles by Stiekema, W. J. Articles by Tobin, E. M.

Articles

Phytochrome Control of the Expression of Two Nuclear Genes Encoding Chloroplast Proteins in Lemna gibba L. G-3 ¹

Department of Biology and Molecular Biology Institute, University of California, Los Angeles, California 90024

Hybridization probes for two nuclear-coded chloroplast proteins of Lemna gibba L. G-3 have been constructed in order to investigate phytochrome regulation of specific sequences. The first probe is a cDNA clone encoding the small subunit of ribulose 1,5-bisphosphate carboxylase. This probe was isolated from a set of Lemna cDNA clones in the bacterial plasmid pBR322. The second probe is a subclone of a genomic clone encoding the light-harvesting chlorophyll a/b-protein. This clone was isolated from a set of genomic clones constructed in the lambda vector Charon 4 with L. gibba DNA fragments generated by partial EcoR1 digestion. The identity of these clones was confirmed by in vitro translation of RNA which hybridized to the cloned DNA. Plants grown under continuous white light contain high concentrations of both RNA sequences; however, when these plants are put into darkness the concentration of these RNAs decreases rapidly relative to the total amount of RNA. Plants grown in the dark with intermittent red light (2 minutes/8 hours) and put into complete darkness for 8 days also contain lower concentrations of the sequences in the total RNA. One minute of red light after this dark period results in a rapid increase in the levels of RNA hybridizing to the probes. The effect of red light can be reversed by far-red light. These experiments demonstrate that phytochrome action can rapidly influence either the rates of transcription or the rates of degradation of these mRNAs.

¹ Supported by National Institutes of Health grant GM-23167 and by the Science and Education Administration of the United States Department of Agriculture under grant 5-901-0410-8-0177-0.

This article has been cited by other articles:

				J. SIMPSON, M. VAN MONTAGU, and L. HERRERA-ESTRELLA Photosynthesis-Associated Gene Families: Differences in Response to Tissue-Specific and Environmental Factors Science, July 4, 1986; 233(4759): 34 - 38. [Abstract] [PDF]

				R. FLUHR, C. KUHLEMEIER, F. NAGY, and N.-H. CHUA Organ-Specific and Light-Induced Expression of Plant Genes Science, May 30, 1986; 232(4754): 1106 - 1112. [Abstract] [PDF]

				E. M. Meyerowitz and R. E. Pruitt Arabidopsis thaliana and Plant Molecular Genetics Science, September 20, 1985; 229(4719): 1214 - 1218. [Abstract] [PDF]