Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
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Plant Physiology 72:1110-1113 (1983)
© 1983 American Society of Plant Biologists

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Articles

Fusion of Plant Protoplasts by Electric Fields

George W. Bates1, John J. Gaynor2 and Narpat S. Shekhawat

Department of Biology, Yale University, New Haven, Connecticut 06511

The electrical fusion technique of Zimmermann and Scheurich (1981 Planta 151: 26-32) has been used to fuse mesophyll protoplasts of Avena, Zea, Vigna, Petunia, and Amaranthus. Electrical fusion proves to be a simple, effective, and general fusion technique that can be controlled to form either dikaryons or large multinucleate fusion bodies. In addition, we show that Vigna mesophyll protoplasts that are subjected to the electrical fields used in this technique are viable in culture. The construction of the fusion chambers, necessary electrical equipment, and the fusion protocol are described in sufficient detail for reproduction of the technique.

1 Present address: Department of Biological Science, Florida State University, Tallahassee, FL 32306.

2 Present address: Laboratory of Plant Molecular Biology, Rockefeller University, New York, NY 10021.






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Copyright © 1983 by the American Society of Plant Biologists