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Plant Physiology 73:175-178 (1983)
© 1983 American Society of Plant Biologists

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Solubilization of the Starch-Granule-Bound Starch Synthase of Normal Maize Kernels 1

Fraser D. Macdonald and Jack Preiss

Department of Biochemistry and Biophysics, University of California, Davis, California 95616

The starch-granule-bound starch synthase from Zea mays has been solubilized with a recovery of between 50 and 84%. Chromatography of the solubilized enzyme on DEAE-Sepharose resolves two fractions of activity which may be distinguished by their response to citrate. Neither solubilized isoenzyme displays any significant activity with UDPglucose.

1 Supported in part by the National Science Foundation Grants PCM 78-16127 and PCM 82-05705.




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P. C. Sehnke, H.-J. Chung, K. Wu, and R. J. Ferl
Regulation of starch accumulation by granule-associated plant 14-3-3 proteins
PNAS, January 16, 2001; 98(2): 765 - 770.
[Abstract] [Full Text] [PDF]


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Copyright © 1983 by the American Society of Plant Biologists