Plant Physiology 73:250-256 (1983)
© 1983 American Society of Plant Biologists
Articles
Organization and Function of Chlorophyll in Membranes of Cyanobacteria during Iron Starvation 1
James A. Guikema2 and
Louis A. Sherman
Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211
Cells of Anacystis nidulans strain R2 and of Synechococcus cedrorum were grown in an iron-deficient medium. Iron starvation induced several pronounced effects without influencing the viability of these cells. The phycocyanin and chlorophyll contents of these cells were depressed, and the absorption maxima of membrane-bound chlorophyll was blue-shifted by 5 nanometers. Cells showed a dramatic increase in original and in maximal chlorophyll fluorescence when monitored at room temperature. Low temperature chlorophyll fluorescence revealed a loss in fluorescence at 696 and 716 nanometers; much of the remaining fluorescence emission was at 686 nanometers. These changes suggest an alteration of membrane composition and structure. This was documented by an electrophoretic analysis of iron-deficient membranes. The prominent findings were: (a) large chlorophyll-protein complexes were not observed in iron-deficient membranes, although the chlorophyll-binding proteins were present; (b) the staining of acrylamide gels with 3,3',5,5'-tetramethylbenzidine plus peroxide indicated that iron deficiency led to a decrease in the quantity of cytochromes. These results support a structural model of the relation between fluorescence and chlorophyll organization in Anacystis. In addition, they suggest a method for studying cytochrome and chlorophyll protein assembly in these membranes.
2 Present address: Division of Biology, Kansas State University, Manhattan, KS 66506.
1 Supported by National Institutes of Health grant GM21827, a NIH Postdoctoral Fellowship (GM07704) to J. A. G., and by the University of Missouri Institutional Biomedical Research Grant RR07053 from NIH. This report represents contribution number 83-161-J of the Kansas Agricultural Experiment Station.
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