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Articles

Reduced Inorganic Carbon Transport in a CO₂-Requiring Mutant of Chlamydomonas reinhardii¹

Department of Agronomy, University of Illinois, Urbana, Illinois 61801, United States Department of Agriculture, Agricultural Research Service, Urbana, Illinois 61801

A mendelian mutant of the unicellular green alga Chlamydomonas reinhardii has been isolated that is deficient in inorganic carbon transport. This mutant strain, designated pmp-1-16-5K (gene locus pmp-1), was selected on the basis of a requirement of elevated CO₂ concentration for photoautrophic growth. Inorganic carbon accumulation in the mutant was considerably reduced in comparison to wild type, and the CO₂ response of photosynthesis indicated a reduced affinity for CO₂ in the mutant. At air levels of CO₂ (0.03-0.04%), O₂ inhibited photosynthesis and stimulated the synthesis of photorespiratory intermediates in the mutant but not in wild type. Neither strain was significantly affected by O₂ at saturating CO₂ concentration. Thus, the primary consequence of inorganic carbon transport deficiency in the mutant was a much lower internal CO₂ concentration compared to wild type. From these observations, we conclude that enzyme-mediated transport of inorganic carbon is an essential component of the CO₂ concentrating system in C. reinhardii photosynthesis.

³ Present address: Départment de Biologie Moléculaire, Université de Genève, CH-1211 Genève 4, Switzerland.

¹ Supported in part by a Rockefeller Foundation postdoctoral fellowship to R. J. S.

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