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Plant Physiology 73:488-496 (1983) © 1983 American Society of Plant Biologists Two Photosynthetic Mechanisms Mediating the Low Photorespiratory State in Submersed Aquatic Angiosperms 1Department of Botany, University of Florida, Gainesville, Florida 32611
The submersed angiosperms Myriophyllum spicatum L. and Hydrilla verticillata (L.f.) Royal exhibited different photosynthetic pulse-chase labeling patterns. In Hydrilla, over 50% of the 14C was initially in malate and aspartate, but the fate of the malate depended upon the photorespiratory state of the plant. In low photorespiration Hydrilla, malate label decreased rapidly during an unlabeled chase, whereas labeling of sucrose and starch increased. In contrast, for high photorespiration Hydrilla, malate labeling continued to increase during a 2-hour chase. Thus, malate formation occurs in both photorespiratory states, but reduced photorespiration results when this malate is utilized in the light. Unlike Hydrilla, in low photorespiration Myriophyllum, 14C incorporation was via the Calvin cycle, and less than 10% was in C4 acids. Ethoxyzolamide, a carbonic anhydrase inhibitor and a repressor of the low photorespiratory state, increased the label in glycolate, glycine, and serine of Myriophyllum. Isonicotinic acid hydrazide increased glycine labeling of low photorespiration Myriophyllum from 14 to 25%, and from 12 to 48% with high photorespiration plants. Similar trends were observed with Hydrilla. Increasing O2 increased the per cent [14C]glycine and the O2 inhibition of photosynthesis in Myriophyllum. In low photorespiration Myriophyllum, glycine labeling and O2 inhibition of photosynthesis were independent of the CO2 level, but in high photorespiration plants the O2 inhibition was competitively decreased by CO2. Thus, in low but not high photorespiration plants, glycine labeling and O2 inhibition appeared to be uncoupled from the external [O2]/[CO2] ratio. These data indicate that the low photorespiratory states of Hydrilla and Myriophyllum are mediated by different mechanisms, the former being C4-like, while the latter resembles that of low CO2-grown algae. Both may require carbonic anhydrase to enhance the use of inorganic carbon for reducing photorespiration.
2 Present address: Department of Agronomy, 1102 South Goodwin Avenue, University of Illinois, Urbana, IL 61801. 3 To whom reprint requests should be addressed. 1 This research was supported by the Science and Education Administration of the United States Department of Agriculture under Grants 78-59-2121-0-1-082-1 and 82-CRCR-1-1147 from the Competitive Research Grants Office. Florida Agricultural Experiment Station Journal Series No. 4494. This article has been cited by other articles:
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