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Plant Physiology 73:534-541 (1983)
© 1983 American Society of Plant Biologists

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Articles

Simultaneous Measurement of Oxygen Evolution and Chlorophyll Fluorescence from Leaf Pieces 1

Thomas J. Delieu and David A. Walker

ARC Research Group on Photosynthesis, Department of Botany, University of Sheffield, Sheffield, S10 2TN, United Kingdom

An apparatus is described which permits the simultaneous measurement of O2 evolution and chlorophyll a fluorescence from illuminated discs or pieces of green leaves. O2 is measured in the gas phase in a temperature-controlled chamber of approximately 5-milliliter capacity. Calibration is effected by injection of air through vents. Response time is approximately 1.5 seconds for O2, and full scale deflection, in normal operating mode, is approximately 10 micromoles O2. The apparatus may also be used to monitor fluorescence alone, in an open mode, in which gas is passed continuously through the chamber.

1 This work was supported by a grant from the Royal Society.




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[Abstract] [Full Text] [PDF]


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Copyright © 1983 by the American Society of Plant Biologists