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Plant Physiology 73:534-541 (1983) © 1983 American Society of Plant Biologists Simultaneous Measurement of Oxygen Evolution and Chlorophyll Fluorescence from Leaf Pieces 1ARC Research Group on Photosynthesis, Department of Botany, University of Sheffield, Sheffield, S10 2TN, United Kingdom
An apparatus is described which permits the simultaneous measurement of O2 evolution and chlorophyll a fluorescence from illuminated discs or pieces of green leaves. O2 is measured in the gas phase in a temperature-controlled chamber of approximately 5-milliliter capacity. Calibration is effected by injection of air through vents. Response time is approximately 1.5 seconds for O2, and full scale deflection, in normal operating mode, is approximately 10 micromoles O2. The apparatus may also be used to monitor fluorescence alone, in an open mode, in which gas is passed continuously through the chamber.
1 This work was supported by a grant from the Royal Society. This article has been cited by other articles:
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