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Plant Physiology 73:576-578 (1983)
© 1983 American Society of Plant Biologists

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Articles

Cell Walls of Phaseolus vulgaris Leaves Contain the Azocoll-Digesting Proteinase 1

Willem van der Wilden2, Johannus H. L. Segers and Maarten J. Chrispeels3

Department of Biology, C-016, University of California at San Diego, La Jolla, California 92093, Lehrstuhl für Pflanzenphysiologie, Ruhr-Universität Bochum, Postfach 102148, D-4630 Bochum, Germany

The leaves of Phaseolus vulgaris L. cv Greensleeves contain an endopeptidase with a pH optimum of 9.0 and an isoelectric point between 10.0 and 10.5. This endopeptidase is the only abundant Azocoll-digesting proteinase in the leaves. The activity of this enzyme is highest in immature leaves and declines as the leaf matures and senesces. Enzymically isolated protoplasts contain very little of this proteinase. The proteinase can be recovered readily from the extracellular fluid obtained by gentle centrifugation of leaf strips vacuum-infiltrated with a buffered solution. These experiments indicate that the Azocoll digesting proteinase is located in the periplasmic space and/or the cell wall.

2 Present address: Gist-Brocades, Department of Cell Biochemistry, Postbus 1, 2600 MA, Delft, The Netherlands.

3 To whom reprint requests should be addressed at Department of Biology, C-016, UCSD, La Jolla, CA 92093.

1 Supported by grants from the Herman Frasch Foundation and the United States Department of Agriculture (Competitive Research Grant Office).




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C. Linnestad, D. N.P. Doan, R. C. Brown, B. E. Lemmon, D. J. Meyer, R. Jung, and O.-A. Olsen
Nucellain, a Barley Homolog of the Dicot Vacuolar-Processing Protease, Is Localized in Nucellar Cell Walls
Plant Physiology, December 1, 1998; 118(4): 1169 - 1180.
[Abstract] [Full Text]


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Copyright © 1983 by the American Society of Plant Biologists