This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (107) Citing Articles via Google Scholar Google Scholar Articles by Watad, A.-E. A. Articles by Lerner, H. R. Search for Related Content PubMed PubMed Citation Articles by Watad, A.-E. A. Articles by Lerner, H. R. Agricola Articles by Watad, A.-E. A. Articles by Lerner, H. R.

Articles

Comparison between a Stable NaCl-Selected Nicotiana Cell Line and the Wild Type ¹

K⁺, Na⁺, and Proline Pools as a Function of Salinity

Department of Botany, The Hebrew University of Jerusalem, 91904, Jerusalem, Israel

An NaCl-resistant line has been developed from suspension-cultured tobacco cells (Nicotiana tabacum/gossii) by stepwise increases in the NaCl concentration in the medium. Resistance showed stability through at least 24 generations in the absence of added NaCl.

Above an external NaCl concentration of 35 millimolar, proline concentration in the selected cells rose steeply with external NaCl, particularly so above 100 millimolar NaCl. Proline accumulation in the wild type was far slighter. Selected cells which had been grown for 24 generations in the absence of added NaCl accumulated proline strongly on re-exposure to NaCl medium, indicating stability of this character. Proline accumulation was fully reversible with a half-time of about 6 hours. When selected cells were transferred sequentially to lower and lower NaCl concentrations, their proline content fell to the level corresponding to the new NaCl concentration. The NaCl-selected cells responded to water stress (i.e. added mannitol) by accumulating markedly more proline than did the wild type.

The addition of Ca²⁺ to the growing and rinsing media minimized Na⁺ and K⁺ binding in the Donnan free space of cell walls and thus allowed assessment of intracellular Na⁺ and K⁺. In both cell types, internal Na⁺ content rose steadily as a function of external NaCl concentration. In the course of 7 days in NaCl media, the wild type cells lost a considerable part of their K⁺ content, the extent of the loss increasing with rise in external NaCl concentration. The selected cells, by contrast, lost no K⁺ at external NaCl concentrations below 50 millimolar external NaCl, and at higher concentrations lost less than the wild type.

This article has been cited by other articles:

				F. Queiros, N. Fontes, P. Silva, D. Almeida, M. Maeshima, H. Geros, and F. Fidalgo Activity of tonoplast proton pumps and Na+/H+ exchange in potato cell cultures is modulated by salt J. Exp. Bot., March 1, 2009; 60(4): 1363 - 1374. [Abstract] [Full Text] [PDF]

				J.-K. Zhu, J. Liu, and L. Xiong Genetic Analysis of Salt Tolerance in Arabidopsis: Evidence for a Critical Role of Potassium Nutrition PLANT CELL, July 1, 1998; 10(7): 1181 - 1192. [Abstract] [Full Text]