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Articles

Ribonucleic Acid and Protein Metabolism in Pea Epicotyls ¹

I. The Aging Process

School of Life Sciences, University of Nebraska, Lincoln, Nebraska 68588-0118

Aging of actively growing, etiolated pea Pisum sativum L. var Alaska plants was initiated by removing the plumules of plants in the third internode stage, and applying lanolin to the cut apices of otherwise intact plants. During the subsequent 4-day aging period, several degenerative events occurred in this apical 10-millimeter region. Ribosomal RNA and messenger RNA contents declined, polyribosomes disaggregated, and the protein synthesizing capacity of the polysomes decreased.

Two-dimensional, silver-stained protein patterns revealed that aging altered the relative amounts of specific cellular proteins accumulated in vivo. In addition, polypeptide patterns generated by cell-free translation of total and polysomal RNA, isolated from unaged and aged tissues, showed major modifications. More than 200 spots could be resolved by two-dimensional gel fluorography of translation products using RNA from fresh tissues. Of these 200 spots, about eight appeared or increased when total RNA from aged tissue was used, and about 58 disappeared or declined. When polysomal RNA from aged tissue was used as template, about 12 spots appeared or increased, whereas about 64 disappeared or decreased. In general, the products which increased after aging were lower molecular weight and those that decreased were higher molecular weight.

³ To whom all correspondence should be addressed.

¹ Supported by United States Department of Agriculture Competitive Research Grants Organization grant No. 59-2311-1-1-689-0 and National Institutes of Health Biomedical Research funds from the University of Nebraska Research Council to E. D.

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