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Plant Physiology 73:822-827 (1983)
© 1983 American Society of Plant Biologists

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Articles

Ribonucleic Acid and Protein Metabolism in Pea Epicotyls 1

III. Response to Auxin in Aged Tissue

Anne Schuster2 and Eric Davies3

School of Life Sciences, University of Nebraska, Lincoln, Nebraska 68588-0118

Applications of auxin to the tips of intact aged pea Pisum sativum L. var Alaska epicotyls resulted in an increase in the content of polyribosomes and poly(A) and in the capacity of isolated polysomes to support protein synthesis in vitro. Few changes were seen in the two-dimensional gel patterns of silver-stained proteins accumulated (or degraded) in vivo even after 15 hours of auxin treatment. In contrast, substantial changes were evident in the two-dimensional gel fluorographs of polypeptides generated in vitro by total RNA and by polysomal RNA from tissue treated with auxin for only 6 hours. Of the 200 spots resolved by fluorography, total RNA from auxin-treated tissue generated 33 spots with increased intensity and 10 with decreased intensity; polysomal RNA yielded 33 spots which increased and only three that decreased. In general, the polypeptides that increased in intensity were higher molecular weight and those that decreased were lower molecular weight. These changes occurred prior to growth and might be prerequisite for the auxin-induced slow growth response seen in this aged tissue.

Comparisons were made between the changes in RNA and protein metabolism occuring during aging and after wounding and auxin treatment of aged tissue. Aging causes a decline in poly(A), polysomes, and protein synthesizing capacity, whereas wounding and auxin treatment cause increases. Wounding appears to act primarily at the level of translation, whereas auxin has a greater effect on transcription. It is argued that the use of excised tissue to study auxin effects on RNA and protein metabolism should be avoided.

2 Present address: Department of Genetics, North Carolina State University, 3515 Gardner Hall, Raleigh, NC 27650.

3 To whom all correspondence should be addressed.

1 Supported by United States Department of Agriculture, Competitive Research Grants Organization grant No. 59-2311-1-1-689-0 and National Institutes of Health Biomedical Research funds from the University of Nebraska Research Council to E. D.






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Copyright © 1983 by the American Society of Plant Biologists