Plant Physiol.
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Plant Physiology 73:1013-1019 (1983)
© 1983 American Society of Plant Biologists

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Articles

Ethylene Production by Auxin-Deprived, Suspension-Cultured Pear Fruit Cells in Response to Auxins, Stress, or Precursor

Rolf Puschmann1 and Roger Romani2

Department of Pomology, University of California, Davis, California 95616

Auxin-deprived, mannitol-supplemented, suspension-cultured pear (Pyrus communis L. Passe Crassane) fruit cells produce large quantities (20-40 nanoliters ethylene per 106 cells per hour) of ethylene in response to auxins, CuCl2 or 1-amino-cyclopropane-1-carboxylic acid (ACC). Maximum rates of production are achieved about 12 hours after the addition of optimal amounts of indoleacetic acid (IAA), naphthalene acetic acid (NAA), 2,4-dichlorophenoxyacetic acid (2,4-D), 4 to 5 hours after the addition of CuCl2 and 1 to 2 hours after the addition of ACC. Supraoptimal concentrations of IAA result in a lag phase followed by a normal response. High concentrations of NAA and 2,4-D result in an early (4-5 hours) stress response and injury.

Continuous protein and RNA synthesis are essential for elaboration of the full IAA response; only protein synthesis is necessary for the response to CuCl2 and ACC. Based on polysomal states and rates of amino acid incorporation, CuCl2 partially inhibits protein synthesis while nonetheless stimulating ethylene production. In general, ethylene production by the pear cells resembles that of other plant systems. Some differences may reflect the sensitivity of the cells and are discussed. The relatively high levels of ethylene produced and the experimental convenience of the cultured cells should make them especially suitable for further investigations of ethylene production and physiology.

1 Permanent address: Departmento de Biologia Vegetal, Universidade Federal de Vicosa, 36570 Vicosa, Minas Gerais, Brasil.

2 To whom reprint requests should be addressed.




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 J Exp BotHome page
J. Lammertyn, C. Franck, B.E. Verlinden, and B.M. Nicolai
Comparative study of the O2, CO2 and temperature effect on respiration between Conference' pear cell protoplasts in suspension and intact pears
J. Exp. Bot., September 1, 2001; 52(362): 1769 - 1777.
[Abstract] [Full Text] [PDF]


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ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 1983 by the American Society of Plant Biologists