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Articles

Sugar-Nucleotide Precursors of Arabinopyranosyl, Arabinofuranosyl, and Xylopyranosyl Residues in Spinach Polysaccharides ¹

Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QW, United Kingdom

Cultured spinach (Spinacia oleracea L. cv Monstrous Viroflay) cells incorporated exogenous L-[³H]arabinose sequentially into -L-arabinopyranose-1-phosphate, uridine diphospho--L-arabinopyranose, uridine diphospho--D-xylopyranose and (in some experiments) -D-xylopyranose-1-phosphate. The amount of ³H in each of these compounds reached a plateau after a few minutes, and could be rapidly chased with nonradioactive L-arabinose, demonstrating rapid turnover. After a few minutes' lag, incorporation of ³H into the arabinofuranosyl, arabinopyranosyl, and xylopyranosyl residues of polysaccharides was linear with respect to time. The kinetics of labeling were compatible with UDP--L-arabinopyranose and UDP--D-xylopyranose being the immediate precursors of arabians (both the pyranose and the furanose residues) and xylans, respectively. No other radioactive nucleotides were formed; in particular, UDP-arabinofuranose was absent. There was no evidence for conversion of arabinopyranose to arabinofuranose within the polysaccharides, suggesting that this conversion occurs during polymer synthesis. The glycolipids detected showed too slow a turnover to be intermediates of pentosan synthesis.

¹ S. C. F. thanks the United Kingdom Science and Engineering Research Council for a Research Fellowship, during tenure of which this work was done.

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