Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
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Plant Physiology 73:915-920 (1983)
© 1983 American Society of Plant Biologists

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Articles

Expression of Zein in Long Term Endosperm Cultures of Maize

Ko Shimamoto1, Marcel Ackermann and Christa Dierks-Ventling

Friedrich Miescher-Institut, P. O. Box 2543, CH-4002 Basel, Switzerland

Continuous cultures, established 10 days after pollination from endosperms of inbred A636 Zea mays (L.) were extracted 21 months later with aqueous ethanol. The solubilized proteins were analyzed by poly-acrylamide-sodium dodecyl sulfate gel electrophoresis. Two protein bands co-migrated with zein, the major storage protein of maize. Immunoblotting of the gel followed by incubation of the immobilized proteins with anti-zein IgG provided evidence that the polypeptides were in fact zein. Electron microscopic studies showed that the cultures contained cells with protein bodies as found in developing endosperms. The protein bodies could be isolated from the cultures and were shown to contain zein. We conclude that the long term cultures described here synthesize zein and deposit it in the form of protein bodies of the type found in developing endosperms. Thus, certain endosperm characteristics and the production of tissue-specific proteins are retained in prolonged culture.

1 Present address: Plantech Research Institute, 1000 Kamoshida, Midori-ku, Yokohama 227, Japan.




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D. Gruis, H. Guo, D. Selinger, Q. Tian, and O.-A. Olsen
Surface Position, Not Signaling from Surrounding Maternal Tissues, Specifies Aleurone Epidermal Cell Fate in Maize
Plant Physiology, July 1, 2006; 141(3): 898 - 909.
[Abstract] [Full Text] [PDF]


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Copyright © 1983 by the American Society of Plant Biologists