This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Guikema, J. A. Articles by Sherman, L. A. Search for Related Content PubMed PubMed Citation Articles by Guikema, J. A. Articles by Sherman, L. A. Agricola Articles by Guikema, J. A. Articles by Sherman, L. A.

Articles

Influence of Iron Deprivation on the Membrane Composition of Anacystis nidulans¹

Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211

Cultures of the cyanobacterium Anacystis nidulans were grown under iron-deficient conditions and then restored by the addition of iron. Membrane proteins from iron-deficient and iron-restored cells were analyzed by lithium dodecyl sulfate-polyacrylamide gradient gel electrophoresis. The incorporation of [³⁵S]sulfate into membrane proteins and lactoperoxidase-catalyzed ¹²⁵I iodination were used to monitor the rates of polypeptide biosynthesis and surface exposure of membrane proteins, respectively. These polypeptide profiles revealed major differences in the membrane composition of iron-deficient and normal cells. Iron deficiency caused a decrease in the amount of certain important membrane proteins, reflecting a decreased rate of biosynthesis of these peptides. Several photosystem II peptides also showed an increase in surface exposure after iron stress. In addition, iron deficiency led to the synthesis of proteins at 34 and 52 kilodaltons which were not present in normal cells. When iron was restored to a deficient culture, a metabolic sequence was initiated within the first 12 h after the addition of iron which led to phenotypically normal cells. Pulse labeling with [³⁵S]sulfate during this period demonstrated that iron addition initiates a coordinated pattern of synthesis that leads to the assembly of normal membranes.

¹ Supported by National Institutes of Health grant GM21827, an N. I. H. Postdoctoral Fellowship (GM07704) to J. A. G., and by the University of Missouri Institutional Biomedical Research Grant RR07053 from N. I. H. This report represents contribution number 84-79J of the Kansas Agricultural Experiment Station.

This article has been cited by other articles:

				T. E. Desquilbet, J.-C. Duval, B. Robert, J. Houmard, and J. C. Thomas In the Unicellular Red Alga Rhodella violacea Iron Deficiency Induces an Accumulation of Uncoupled LHC Plant Cell Physiol., November 15, 2003; 44(11): 1141 - 1151. [Abstract] [Full Text] [PDF]

				A. K. Singh, L. M. McIntyre, and L. A. Sherman Microarray Analysis of the Genome-Wide Response to Iron Deficiency and Iron Reconstitution in the Cyanobacterium Synechocystis sp. PCC 6803 Plant Physiology, August 1, 2003; 132(4): 1825 - 1839. [Abstract] [Full Text] [PDF]

				T. S. Bibby, J. Nield, and J. Barber Three-dimensional Model and Characterization of the Iron Stress-induced CP43'-Photosystem I Supercomplex Isolated from the Cyanobacterium Synechocystis PCC 6803 J. Biol. Chem., November 9, 2001; 276(46): 43246 - 43252. [Abstract] [Full Text] [PDF]

				A. K. Singh and L. A. Sherman Identification of Iron-Responsive, Differential Gene Expression in the Cyanobacterium Synechocystis sp. Strain PCC 6803 with a Customized Amplification Library J. Bacteriol., June 15, 2000; 182(12): 3536 - 3543. [Abstract] [Full Text]