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Plant Physiology 74:795-799 (1984)
© 1984 American Society of Plant Biologists

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Articles

De Novo Maltotriose Biosynthesis from the Reducing End by Spinacia oleracea L. Chloroplasts

James C. Linden1 and N. Schilling

Department of Biochemistry and Biophysics, Iowa State University, Ames, Iowa 50011, Botanisches Institut der Universitaet Muenchen, 8000 Muenchen 19, Federal Republic of Germany

The distribution of 14C in the various glucose residues of maltotriose was studied as a function of time of photosynthesis of isolated chloroplasts of spinach (Spinacia oleracea L.) using 14CO2. The distribution of label showed that the reducing-end glucose residue was labeled first and the label subsequently distributed to the second and third glucose residues at approximately equal rates.

A mechanism for the distribution of label and the synthesis of maltotriose from the reducing end is presented. The mechanism has postulated to be the same as that for the maltose synthase recently described by Schilling. Maltose biosynthesis from {alpha}-D-glucose-1-phosphate was characterized as involving two glucosyl intermediates by a double displacement mechanism with inversion of configuration. The mode of enzymic action by which maltosyl intermediates were transferred to glucosyl intermediates was consistent with the fractional distribution of radioactivity found in each glucose unit of maltotriose during short term photosynthesis experiments.

1 Supported by the Alexander von Humboldt Stiftung, Bad Godesberg, Federal Republic of Germany during the portion of the work conducted at the University of Munich. Current address: Department of Agricultural and Chemical Engineering, Colorado State University, Fort Collins, CO 80523.






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