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Plant Physiology 74:810-814 (1984)
© 1984 American Society of Plant Biologists

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Articles

Abscisic Acid Metabolism by Source and Sink Tissues of Sugar Beet 1

Jaleh Daie2, Roger Wyse, Mich Hein3 and Mark L. Brenner

United States Department of Agriculture, Agriculture Research Service, Crops Research Laboratory, Utah State University, UMC 63, Logan, Utah 84322, Department of Horticulture Science and Landscape Architecture, University of Minnesota, St. Paul, Minnesota 55108

The fate of exogenously applied, labeled abscisic acid (±)-(ABA) was followed in source leaves and taproot sink tissues of sugar beet (Beta vulgaris cv AH-11). The objective was to determine if differential pathways for ABA metabolism exist in source and sink tissues. Tissue discs were incubated for up to 13 hours in a medium containing 1 micromolar labeled ABA. At various time intervals, samples were taken for metabolite determination by reverse-phase high performance liquid chromatography. The labeled metabolites were identified by retention times using an online scintillation counter.

Dihydrophaseic acid (DPA) aldopyranoside, DPA, phaseic acid (PA), ABA glucose ester (ABA-GE), and two unidentified compounds were recovered from both tissues. An additional unidentified metabolite was also present in root tissue. Leaf tissue discs exhibited a higher capacity for ABA conjugation, and root discs showed a greater preference for ABA catabolism to PA and DPA. After 4 to 5 hours, ABA incorporation into the various metabolites was proportional to the external ABA concentration in both tissues. But the internal ABA pool size was independent of external concentrations below 10–6 molar. These results suggested that rates of ABA metabolism was proportional to the rates of uptake in both tissues.

2 Present address: Biology Department, UMC 63, Utah State University, Logan, UT 84322.

3 Present address: Monsanto Agricultural Products Co., 800 N. Lindbergh Bldg., St. Louis, MO 63166.

1 Supported in part by the Science and Education Administration of the United States Department of Agriculture under Grant 5901-0410-8-0187-0 from the Competitive Grants Office. Cooperative investigations of Agriculture Research Service. United States Department of Agriculture, and the Utah State Agricultural Experiment Station. Approved as Journal Paper No. 2318, Utah Agricultural Experiment Station, Logan, UT 84322.






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Copyright © 1984 by the American Society of Plant Biologists