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Plant Physiology 74:944-946 (1984)
© 1984 American Society of Plant Biologists

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Heat Stress Responses in Cultured Plant Cells 1

Effect of Culture Handling and Age

Min-Tze Wu, Stephen J. Wallner and John W. Waddell

Department of Horticulture, Colorado State University, Fort Collins, Colorado 80523

The pipetting of pear (Pyrus communis cv Bartlett) suspension cultures was followed by a substantial but transient decrease in heat sensitivity. During a culture cycle, pear cells were most sensitive to heat at day 3, which coincided with the period of most active cell division. To minimize serious artifacts, the influence of culture handling and age on parameters such as heat sensitivity must be standardized.

1 Supported in part by a grant from the Faculty Council Committee on Research, Colorado State University. Published with the approval of the Colorado Agricultural Experiment Station as Scientific Series Paper No. 2866.






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Copyright © 1984 by the American Society of Plant Biologists