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Plant Physiology 74:984-988 (1984)
© 1984 American Society of Plant Biologists

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Articles

Reduction of Ferric Leghemoglobin in Soybean Root Nodules 1

Keuk-Ki Lee and Robert V. Klucas

Department of Agricultural Biochemistry, University of Nebraska-Lincoln, Lincoln, Nebraska 68583-0718

Reduction of ferric leghemoglobin to ferrous leghemoglobin in soybean nodules (Glycine max [L.] Merr. cv Woodworth) was studied using a spectrophotometer equipped with an in-cell space diffuse reflectance accessory. Nodule slices prepared and scanned under nitrogen gas showed a ferrous leghemoglobin absorption spectrum. Nodule slices equilibrated with 100% O2 or air exhibited two absorption bands characteristic of oxygenated leghemoglobin. The addition of CO shifted those bands to CO leghemoglobin absorption bands. Potassium ferricyanide was not effective in oxidizing ferrous to ferric leghemoglobin in nodule slices. However, ferric leghemoglobin was formed by treating the nodule slices with hydroxylamine, and this was confirmed by complexing the ferric leghemoglobin to acetate, fluoride, or nicotinic acid. The diminution of ferric leghemoglobin was monitored as a function of time, and in the presence of nicotinic acid, the conversion of ferric to ferrous leghemoglobin was monitored by the appearance of ferrous leghemoglobin nicotinate complex as a function of time. Ferric leghemoglobin reduction was also confirmed by direct transmission spectrophotometry. The evidence presented here suggests that ferrileghemoglobin reduction occurs in nodule slices.

1 This material is based upon work supported by the United States Department of Agriculture under Agreement No. 59-2311-1-1-673-0. Published as Paper No. 7322, Journal Series, Nebraska Agricultural Experiment Station.




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J. F. Moran, Z. Sun, G. Sarath, R. Arredondo-Peter, E. K. James, M. Becana, and R. V. Klucas
Molecular Cloning, Functional Characterization, and Subcellular Localization of Soybean Nodule Dihydrolipoamide Reductase
Plant Physiology, January 1, 2002; 128(1): 300 - 313.
[Abstract] [Full Text] [PDF]


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Copyright © 1984 by the American Society of Plant Biologists