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Plant Physiology 75:131-137 (1984)
© 1984 American Society of Plant Biologists

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Articles

Conditions Required for the Rapid Activation In Vitro of the Chloroplast Fructose-1,6-bisphosphatase

Luciana Rosa1 and F. Robert Whatley

Botany School, University of Oxford, South Parks Road, Oxford OX1 3RA, United Kingdom

Conditions required for the reductive activation of purified, spinach chloroplast fructose-1,6-bisphosphatase (EC 3.1.3.11) have been determined in vitro. Full reductive activation was observed only when fructose-1,6-bisphosphate and Mg2+ were present at the same time as the reducing agent (dithiothreitol). Reduction in the absence either of fructose-1,6-bisphosphate or of Mg2+ slowly and irreversibly inactivated the enzyme. The concentration of fructose-1,6-bisphosphate that must be present during reduction for maximum activation depends upon the divalent cation present: it is highest with Mg2+, lower with Ca2+, and lowest when both Mg2+ and Ca2+ are present. A scheme for the reductive activation and inactivation of the enzyme is presented.

1 Permanent address: Istituto di Botanica dell'Università di Napoli, Via Foria 223, Naples, Italy.






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Copyright © 1984 by the American Society of Plant Biologists