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Changes in Levels of -Amylase Components in Barley Tissues during Germination and Early Seedling Growth ¹

Grain Research Laboratory, Canadian Grain Commission, Winnipeg, Manitoba R3C 3G8 Canada, Laboratoire de Physiologie des Organes Végétaux après Recolte, Centre National de la Recherche Scientifique, 92190 Meudon, France

Kernels of Klages barley (Hordeum vulgare L.) were germinated for 1 to 4 days on moist sand at 18°C. Representative kernels from each time period were dissected to give the following fractions: scutellum, subscutellar endosperm, aleurone-scutellum interface, remaining aleurone, subaleurone endosperm, and core endosperm. These tissues were analyzed for -amylase components by isoelectric focusing and rocket-line immunoelectrophoresis. Although aleurone and scutellar tissues appeared to synthesize the same -amylase components, enzyme was detected first in the scutellum. A larger proportion of scutellar -amylase was excreted into the endosperm compared to aleurone synthesized -amylase. Aleurone cells appeared to synthesize appreciably more -amylase than did scutellar tissue.