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Plant Physiology 75:369-371 (1984)
© 1984 American Society of Plant Biologists

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Articles

Regulation of the Shikimate Pathway of Carrot Cells in Suspension Culture 1

Joann A. Suzich, Raoul Ranjeva2, Paul M. Hasegawa and Klaus M. Herrmann

Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907, Department of Horticulture, Purdue University, West Lafayette, Indiana 47907

The activity of the first enzyme of the shikimate pathway, 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase, is demonstrated in extracts of Daucus carota cells grown in suspension culture. Maximum specific enzyme activity is found midway through the logarithmic growth of the culture; cells in lag and stationary phases of growth have lower enzyme levels. The enzyme is activated by tyrosine and tryptophan. The extent of activation varies during cell growth.

2 Present address: Centre de Physiologie Vegetale, Universite Paul Sabatier, Toulouse, France.

1 Supported by a David Ross Fellowship to J. A. S. Journal Paper No. 9620 of the Purdue University Agricultural Experiment Station.






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Copyright © 1984 by the American Society of Plant Biologists