Plant Physiol.
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Plant Physiology 75:508-510 (1984)
© 1984 American Society of Plant Biologists

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A Rapid, Sensitive Method for Quantitating Subunits in Purified Ribulose Bisphosphate Carboxylase Preparations 1

T. John Andrews and Beth Ballment

Australian Institute of Marine Science, PMB No. 3, TMC, Townsville, Australia 4810

Studies of the interactions of the large and small subunits of ribulose bisphosphate carboxylase require a knowledge of the concentrations of the subunits present in various preparations and their ratio. Since existing sodium dodecyl sulfate-gel electrophoresis procedures proved quantitatively unreliable, a technique based on high performance-gel filtration was developed. The latter is most reliable, takes only about 30 minutes to perform, and detects a minimum of 0.25 micrograms of each subunit.

1 Contribution No. 230 from the Australian Institute of Marine Science.






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ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 1984 by the American Society of Plant Biologists