Plant Physiol.
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Plant Physiology 75:662-664 (1984)
© 1984 American Society of Plant Biologists

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Articles

Localization of Mg-Chelatase and Mg-Protoporphyrin IX Monomethyl Ester (Oxidative) Cyclase Activities within Isolated, Developing Cucumber Chloroplasts 1

Thomas P. Fuesler, Yum-Shing Wong and Paul A. Castelfranco

Department of Botany, University of California, Davis, California 95616

Magnesium chelatase and magnesium protoporphyrin IX monomethyl ester (oxidative) cyclase activities were both sensitive to inhibition by p-chloromercuribenzoate in intact, developing cucumber (Cucumis sativus L. var Beit Alpha) chloroplasts. Magnesium chelatase was also sensitive to the membrane-impermeable mercurial p-chloromercuribenzene sulfonate (PCMBS), while cyclase activity was only slightly sensitive. When the plastids were pretreated with PCMBS, triosephosphate dehydrogenase activity was inhibited very slightly, indicating that PCMBS does not readily penetrate through the chloroplast envelope. These results suggest that magnesium chelatase is located in the chloroplast envelope, while the cyclase is located deeper within the chloroplast.

1 Supported by National Science Foundation Grant PCM 81-08538.




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Copyright © 1984 by the American Society of Plant Biologists