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Plant Physiology 75:700-704 (1984)
© 1984 American Society of Plant Biologists

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Articles

In Vitro Fatty Acid Synthesis and Complex Lipid Metabolism in the Cyanobacterium, Anabaena Variabilis1

II. Acyl Transfer and Complex Lipid Formation

Nora W. Lem2 and Paul K. Stumpf

Department of Biochemistry & Biophysics, University of California, Davis, California 95616

In vitro fatty acid transfer to form complex lipids was observed in crude cell extracts of Anabaena variabilis using [1-14C]palmitoyl-acyl carrier protein, [1-14C]stearoyl-acyl carrier protein, and [1-14C]oleoyl-acyl carrier protein substrates. The data indicated that there was a rapid transfer of the fatty acids into the complex lipids. The greatest amount of radioactivity was observed in the monogalactosyl diacylglycerol fractions and there appeared to be a preference for the transfer of stearate over palmitate. The exogenously added lysophospholipids, (lysophosphatidylglycerol, lysophosphatidylcholine) and 2-monopalmitin acted as acceptors in acyl transfer. Addition of the hypolipidemic drug, WY14643, inhibited the fast acyl transfer reaction and showed that the first product of acyl transfer was diglyceride followed by monogalactosyl diacylglycerol. Thioesters of Coenzyme A do not seem to be involved in these reactions.

2 Recipient of a North Atlantic Treaty Organization Postdoctoral Science Fellowship awarded by the Natural Sciences and Engineering Research Council of Canada. Permanent address: Biology Department, University of Waterloo, Waterloo, Ontaria N2L, 3G1, Canada.

1 Supported in part by National Science Foundation grant PCM79-03976.




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K. Okazaki, N. Sato, N. Tsuji, M. Tsuzuki, and I. Nishida
The Significance of C16 Fatty Acids in the sn-2 Positions of Glycerolipids in the Photosynthetic Growth of Synechocystis sp. PCC6803
Plant Physiology, June 1, 2006; 141(2): 546 - 556.
[Abstract] [Full Text] [PDF]


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Copyright © 1984 by the American Society of Plant Biologists