This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Anderson, M. A. Articles by Clarke, A. E. Search for Related Content PubMed PubMed Citation Articles by Anderson, M. A. Articles by Clarke, A. E. Agricola Articles by Anderson, M. A. Articles by Clarke, A. E.

Articles

A High Proportion of Hybridomas Raised to a Plant Extract Secrete Antibody to Arabinose or Galactose

Plant Cell Biology Research Centre, School of Botany, University of Melbourne, Parkville Victoria 3052, Australia, Research Centre for Cancer and Transplantation, Department of Pathology, University of Melbourne, Parkville Victoria 3052, Australia

A high proportion of hybridomas, obtained from mice immunized with style extracts prepared from mature flowers of an ornamental tobacco, Nicotiana alata, secrete antibody to arabinogalactan protein (AGP). The specificity of the antibodies secreted by three cloned cell lines is primarily directed to -D-galactopyranose and -L-arabinofuranose; antibodies from two cell lines preferentially bind -D-galactopyranose residues and antibodies from the other cell line preferentially bind -L-arabinofuranose. As AGPs are components of most plant tissues and exudates, it is likely that attempts to raise monoclonal antibodies to other plant extracts will result in hybridomas producing antibodies to AGPs.

This article has been cited by other articles:

				R. Leonard, B. O. Petersen, M. Himly, W. Kaar, N. Wopfner, D. Kolarich, R. van Ree, C. Ebner, J. O. Duus, F. Ferreira, et al. Two Novel Types of O-Glycans on the Mugwort Pollen Allergen Art v 1 and Their Role in Antibody Binding J. Biol. Chem., March 4, 2005; 280(9): 7932 - 7940. [Abstract] [Full Text] [PDF]