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Articles

Sugar Transport into Protoplasts Isolated from Developing Soybean Cotyledons ¹

I. Protoplast Isolation and General Characteristics of Sugar Transport

Central Research and Development Department, Experimental Station, E. I. du Pont de Nemours and Company, Wilmington, Delaware 19898

A procedure is described to isolated functional protoplasts from developing soybean (Glycine max L. Merr. cv Wye) cotyledons. Studies of sucrose and hexose uptake into these protoplasts show that the plasmalemma of cotyledons during the stage of rapid seed growth contains a sucrose-specific carrier which is energetically and kinetically distinct from the system(s) involved in hexose transport. For example, sucrose, but not hexose uptake: (a) is inhibited by alkaline pH and the nonpermeant SH modifier, p-chloromercuribenzene sulfonic acid; (b) is stimulated by fusicoccin; (c) shows both a saturable and a linear component of uptake in response to substrate concentration; and (d) displays a sharp temperature response (high Q₁₀ value and high activation energies).

¹ Contribution No. 3340 from Central Research and Development Department, Experimental Station, E. I. du Pont de Nemours and Company, Wilmington, DE 19898. The protoplast isolation procedure and preliminary results of sucrose uptake have been reported at the Annual Meeting of the American Society of Plant Physiologists at Colorado State University, Fort Collins, CO, August, 1983.

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