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Plant Physiology 76:103-105 (1984)
© 1984 American Society of Plant Biologists

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Articles

A Simple Plant Nutrient Solution Purification Method for Effective Removal of Trace Metals Using Controlled Pore Glass-8-Hydroxyquinoline Chelation Column Chromatography

David L. Eskew1, Ross M. Welch and Earle E. Cary

United States Plant, Soil and Nutrition Laboratory, Agricultural Research Service, United States Department of Agriculture, Cornell University, Ithaca, New York 14853, Department of Agronomy, Cornell University, Ithaca, New York 14853

Column chelation chromatography on controlled pore glass-8-hydroxyquinoline was demonstrated to be a very efficient method for removing trace metal contaminants from concentrated macronutrient salt solutions used to prepare nutrient media. By using 63Ni and 65Zn radio-isotopes as tracers, controlled pore glass-8-hydroxyquinoline column packings were found to retain 99.9% of the radiotracer and quantitative recovery of the radioisotopes from these columns was obtained by eluting with 1.2 N HCl. This method has several advantages over liquid-liquid extraction methods of purification which previously have been used in plant micronutrient research.

1 Present address: International Atomic Energy Agency, Wagramerstrasse 5, PO Box 200, A-1400 Vienna, Austria.






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Copyright © 1984 by the American Society of Plant Biologists