Plant Physiol. Drug Metab Dispos
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Plant Physiology 76:21-25 (1984)
© 1984 American Society of Plant Biologists

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Adenine Nucleotide Regulation of Malate Oxidation in Isolated Mung Bean Hypocotyl Mitochondria 1

Alyson K. Tobin2 and Curtis V. Givan

Department of Plant Biology, University of Newcastle-upon-Tyne, Newcastle-upon-Tyne NE1 7RU England

We have studied the effects of ATP and ADP on the oxidation of malate by coupled and uncoupled mitochondria prepared from etiolated hypocotyls of mung bean (Vigna radiata L.).

In coupled mitochondria, ATP (1 millimolar) increased pyruvate production and decreased oxaloacetate formation without altering the rate of oxygen consumption. ATP also significantly decreased oxaloacetate production and increased pyruvate production in mitochondria that were uncoupled by carbonyl cyanide p-trifluoromethoxyphenyl hydrazone plus oligomycin.

In coupled mitochondria, ADP (1 millimolar) increased the production of both pyruvate and oxaloacetate concomitantly with the acceleration of oxygen uptake to the state 3 rate. The effects of ADP were largely eliminated in uncoupled mitochondria. These results indicate that, whereas the ADP stimulation of oxaloacetate and pyruvate production in the coupled mitochondria is brought about primarily as the result of the accelerated rates of electron transport and NADH oxidation by the respiratory chain in state 3, ATP has significant regulatory effects independent of those that might be exerted by control of electron transport.

2 Present address: Biochemistry Department, University of Sussex, Falmer, Brighton, Sussex BN1 9QG England.

1 Supported by a postgraduate research studentship from the Science and Engineering Research Council (U.K.) to A. K. T.






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