Plant Physiol.
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Plant Physiology 76:26-30 (1984)
© 1984 American Society of Plant Biologists

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Articles

Characterization of the Solubilized Plasma Membrane ATPase of Red Beet 1

Donald P. Briskin2 and Ronald J. Poole

Department of Biology, McGill University, Montreal, Quebec H3A 1B1 Canada

The plasma membrane ATP-phosphohydrolase (ATPase) from red beet (Beta vulgaris L.) storage tissue was solubilized with the zwitterionic detergent Zwittergent 3-14 from a plasma membrane-enriched fraction which was extracted with the anionic detergent, sodium deoxycholate. For both the extraction of extraneous proteins by deoxycholate and the solubilization of active plasma membrane ATPase by Zwittergent 3-14, the optimal concentration of detergent was 0.1% (weight per volume) with a detergent to protein ratio of 1.0 (milligram per milligram). The properties of the solubilized ATPase were found to be similar to the membrane-bound enzyme with respect to pH optimum, substrate specificity, inhibitor sensitivity, and kinetics of K+ stimulation. The solubilized ATPase preparation formed a rapidly turning over phosphoenzyme, the breakdown velocity of which was increased in the presence of 50 millimolar KCl. Solubilization with 0.1% Zwittergent 3-14 following extraction with 0.1% deoxycholate resulted in an increase in both ATPase activity and steady state phosphoenzyme level; however, a direct correspondence between the increase in ATPase activity and phosphorylation level did not exist. It is proposed that this discrepancy may be the result of a detergent-mediated modification of kinetic rate constants in the mechanism of the enzyme.

2 Present Address: Plant Biochemistry and Bioregulation Laboratory, USDA-ARS, Utah State University, UMC 63, Logan, UT 84322.

1 Supported by the Natural Sciences and Engineering Research Council of Canada and the Department of Education of Quebec.






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Copyright © 1984 by the American Society of Plant Biologists