This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Aspart, L. Articles by Penon, P. Search for Related Content PubMed PubMed Citation Articles by Aspart, L. Articles by Penon, P. Agricola Articles by Aspart, L. Articles by Penon, P.

Articles

Developmental Regulation of the Synthesis of Proteins Encoded by Stored mRNA in Radish Embryos ¹

Laboratoire de Physiologie Végétale, E.R.A. No. 226 du CNRS, Université de Perpignan, 66025 Perpignan-Cedex, France

Major radish (Raphanus sativus L. cv National) proteins synthesized at the beginning of germination have been characterized by their migration in two-dimensional electrophoresis.

The use of 15-minute labelings shows that these proteins are encoded by stored mRNA. They undergo little or no posttranslational modification. Other proteins become detectable only after 1 hour of imbibition, and are probably encoded by newly synthesized mRNA. Comparison with proteins synthesized during embryogenesis, late germination, or with those present in dry embryos allows the classification of the proteins encoded by stored mRNA into two sets:

The first set is synthesized also during late embryogenesis and is present in dry embryos. Some of these polypeptides are no longer synthesized later in germination while the others continue to be synthesized. The corresponding stored mRNAs can be considered as remnants of mRNA actively translated during embryogenesis.

The second set is synthesized only during early germination. Their messengers appear during late embryogenesis although they are apparently not translated at this stage, but translation can be induced by a desiccation treatment. These polypeptides may play a particular role during early germination.

This article has been cited by other articles:

				L. Rajjou, Y. Lovigny, S. P.C. Groot, M. Belghazi, C. Job, and D. Job Proteome-Wide Characterization of Seed Aging in Arabidopsis: A Comparison between Artificial and Natural Aging Protocols Plant Physiology, September 1, 2008; 148(1): 620 - 641. [Abstract] [Full Text] [PDF]

				J. Catusse, J.-M. Strub, C. Job, A. Van Dorsselaer, and D. Job Proteome-wide characterization of sugarbeet seed vigor and its tissue specific expression PNAS, July 22, 2008; 105(29): 10262 - 10267. [Abstract] [Full Text] [PDF]