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Plant Physiology 76:854-857 (1984)
© 1984 American Society of Plant Biologists

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Diffusion of Gases through Plant Tissues 1

Entry of Acetylene into Legume Nodules

Lawrence C. Davis

Department of Biochemistry, Kansas State University, Manhattan, Kansas 66506

I have measured acetylene diffusion through plant tissues including nodules from several species of legume—vetch, peas, soybeans, and Sesbania rostrata. The observed half-time for reequilibration of internal and external concentration is less than 1 minute for typical nodules. Inward diffusion of acetylene in air is rapid relative to the use of acetylene by nitrogenase so that diffusion of acetylene would not be a significant limiting factor for nitrogenase activity in air. However, under an atmosphere of Ar:O2 where there is no N2 reduction, the inward diffusion rate of acetylene into larger nodules could produce a measurable limitation of observed nitrogenase activity at low acetylene concentrations.


1 Supported by the Kansas Agricultural Experiment Station and a grant from the United States Department of Agriculture No. 83-CR-1-1301. This is Contribution No. 84-418-J from the Kansas Agricultural Experiment Station.







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