Plant Physiology 76:865-870 (1984)
© 1984 American Society of Plant Biologists
Articles
Mechanism of Amino Acid Uptake by Sugarcane Suspension Cells 1
Roger E. Wyse2 and
Ewald Komor
USDA-ARS, Crops Research Laboratory, Utah State University, Logan, Utah 84322,
Botanisches Institute Der Universitat Bayreuth, Lehrstuhl Pflanzenphysiologie, Universitatsstrasse 30, D-8580 Bayreuth, West Germany
The amino acid carriers in sugarcane suspension cells were characterized for amino acid specificity and the stoichiometry of proton and potassium flux during amino acid transport.
Amino acid transport by sugarcane cells is dependent upon three distinct transport systems. One system is specific for neutral amino acids and transports all neutral amino acids including glutamine, asparagine, and histidine. The uptake of neutral amino acids is coupled to the uptake of one proton per amino acid; one potassium ion leaves the cells for charge compensation. Histidine is only taken up in the neutral form so that deprotonation of the charged imidazole nitrogen has to occur prior to uptake. The basic amino acids are transported by another system as uniport with charge-compensating efflux of protons and potassium. The acidic amino acids are transported by a third system. Acidic amino acids bind to the transport site only if the distal carboxyl group is in the dissociated form (i.e. if the acidic amino acid is anionic). Two protons are withdrawn from the medium and one potassium leaves the cell for charge compensation during the uptake of acid amino acids. Common to all three uptake systems is a monovalent positively charged amino acidproton carrier complex at the transport site.
2 Recipient of a grant from Organization for Economic Cooperation and Development, which allowed the stay in Bayreuth.
1 Supported by a grant from Deutsche Forschungsgemeinschaft to E.K.
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