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Plant Physiology 76:984-988 (1984)
© 1984 American Society of Plant Biologists

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Articles

Tissue Cultures Derived from Ineffective Root Nodules of Alfalfa 1

Callus Initiation and Enzymic Comparisons

Carroll P. Vance, Lois E. B. Johnson and Kristin L. M. Boylan2

United States Department of Agriculture, Agricultural Research Service, University of Minnesota, St. Paul, Minnesota 55108, Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, Minnesota 55108

Callus tissue cultures were developed from apical meristem regions of tumor-like ineffective root nodules of alfalfa. Callus growth was a function of tissue source and hormone composition and concentration. Callus derived from ineffective nodules also were shown not to contain Rhizobium meliloti.

Glutamate dehydrogenase, glutamine synthetase, glutamate synthase, glutamate oxaloacetate transaminase and phosphoenolpyruvate carboxylase activities were present in callus cultures and in the respective nodule source used for callus induction. The mean specific activity of all enzymes evaluated was higher in callus cultures than in ineffective nodules. Quantitative but not qualitative differences in enzyme activities were evident between ineffective nodules and callus derived from these nodules. Tissue cultures derived from ineffective nodules may provide a model system to evaluate host plant-Rhizobium interactions.

2 Present address: Agricultural Chemicals Department, E. I. Dupont de Nemours and Co., Newark, Wilmington, DE 19711.

1 Joint contribution Department of Agronomy and Plant Genetics, Minnesota Agricultural Experimental Station and United States Department of Agriculture, Agricultural Research Service. This research was supported in part by United States Department of Agriculture-Competitive Research Grants Office Grant 83-CRCR-1-1304. Paper No. 13,983, Scientific Journal Series, Minnesota Agricultural Experimental Station.






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Copyright © 1984 by the American Society of Plant Biologists