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Plant Physiology 77:74-78 (1985)
© 1985 American Society of Plant Biologists

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Acclimation to Low Temperature by Microsomal Membranes from Tomato Cell Cultures

Frances M. Dupont1 and J. Brian Mudd

ARCO Plant Cell Research Institute, 6560 Trinity Court, Dublin, California 94568

Sealed vesicles were prepared from microsomal membranes from cell suspension cultures of tomato (Lycopersicon esculentum Mill cv VF36). ATP-dependent proton transport activity by the vesicles was measured as quenching of fluorescence of acridine orange. Measurements of proton transport were correlated with the activity of a nitrate-inhibitable ATPase. The initial rate of proton influx into the vesicles was strongly temperature dependent with a Q10 of 2 and a maximum rate near 35°C. The data suggest that passive permeability did not increase at chilling temperatures but did increase rapidly with temperatures above 30°C. A comparison was made between membranes from cell cultures grown at 28°C and 9°C. The temperature optimum for proton transport broadened and shifted to a lower temperature range in membranes from cells maintained at 9°C.

1 Present address: USDA Western Regional Laboratory, 800 Buchannan Street, Albany, CA 94710.






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Copyright © 1985 by the American Society of Plant Biologists