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Plant Physiology 77:318-320 (1985) © 1985 American Society of Plant Biologists Metabolic Interactions between Spinach Leaf Nitrite Reductase and Ferredoxin-NADP ReductaseCompetition for Reduced FerredoxinLight and Plant Growth Laboratory, Plant Physiology Institute, Beltsville, Maryland 20705, USDA/ARS, Beltsville Agricultural Research Center-West, Beltsville, Maryland 20705 Steady state rates of NADP reduction decline upon commencement of nitrite reduction in reconstituted chloroplast preparations. Similarly, steady state rates of nitrite reduction are lower, but not zero, during concurrent NADP reduction. These results imply that competition for substrate occurs and suggest that nitrite reduction can successfully compete for reduced ferredoxin, even at high rates of NADP reduction.
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