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Plant Physiology 77:339-345 (1985)
© 1985 American Society of Plant Biologists

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Articles

Isolation and Antigenic Characterization of Corn Mitochondrial F1-ATPase 1

Vitaly L. Spitsberg, Nancy E. Pfeiffer, Bruce Partridge, Dwane E. Wylie and Sheldon M. Schuster

Plant Breeding and Biometry, Cornell University, Ithaca, New York, Department of Chemistry, University of Nebraska, Lincoln, Nebraska 68588-0304, School of Biological Sciences, University of Nebraska, Lincoln, Nebraska 68588-0304

Corn mitochondrial F1-ATPase was purified from submitochondrial particles by chloroform extraction. Enzyme stored in ammonium sulfate at 4°C was substantially activated by ATP, while enzyme stored at –70°C in 25% glycerol was not. Enzyme in glycerol remained fully active (8-9 micromoles Pi released per minute per milligram), while the ammonium sulfate preparations steadily lost activity over a 2-month storage period. The enzyme was cold labile, and inactived by 4 minutes at 60°C. Treatment with octylglucoside resulted in complete loss of activity, while vanadate had no effect on activity. The apparent subunit molecular weights of corn mitochondrial F1-ATPase were determined by SDS-polyacrylamide gel electrophoresis to be 58,000 ({alpha}), 55,000 (beta), 35,000 ({gamma}), 22,000 ({delta}), and 12,000 ({varepsilon}). Monoclonal and polyclonal antibodies used in competitive binding assays demonstrated that corn mitochondrial F1-ATPase was antigenically distinct from the chloroplastic CF1-ATPases of corn and spinach. Monoclonal antibodies against antigenic sites on spinach CF1-ATPase beta and {gamma} subunits were used to demonstrate that those sites were either changed substantially or totally absent from the mitochondrial F1-ATPase.

1 N. P. was supported by National Institutes of Health, National Research Service Award CA 07420 from the National Cancer Institute. V. S. was supported by National Science Foundation Grant PCM-8341774. S. M. S. acknowledges support from the National Science Foundation Grant PCM 84-09287, as well as a Research Career Development Award from the National Cancer Institute (CA 00628) of the Department of Health and Human Services.






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ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 1985 by the American Society of Plant Biologists