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Glutamine Metabolism in Corn Kernels Cultured In Vitro¹

Biology Department, McMaster University, Hamilton, Ontario, Canada L8S 4K1

The fate of glutamine, the major source of nitrogen supplied to the developing maize endosperm, has been examined in endosperm tissues of corn caryopsis grown under sterile conditions. In the culture system, [U-¹⁴C]glutamine was included in the medium or was injected directly into the endosperm. Samples were harvested at intervals up to 168 hours. Protein and starch fractions were then separated and analyzed for their ¹⁴C content. At 168 hours, 31% of the total label incorporated in the endosperm was in zein, 15% in glutelin, and 24% in starch. When individual amino acids and sugars in the endosperm powders were analyzed, the ¹⁴C still remaining in the glutamine accounted for only 12 to 14% of the total radioactivity.

¹ Supported by research grants from National Sciences and Engineering Research Council-Canada (A2818).

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