Plant Physiol.
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Plant Physiology 77:847-850 (1985)
© 1985 American Society of Plant Biologists

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An Analysis of Growth Regulator Interactions and Gene Expression during Auxin-Induced Cell Elongation Using Cloned Complementary DNAs to Auxin-Responsive Messenger RNAs 1

John C. Walker, Jolanta Legocka2, Leonard Edelman and Joe L. Key

Department of Botany, The University of Georgia, Athens, Georgia 30602, Department of Biochemistry, The University of Georgia, Athens, Georgia 30602

We have examined the effects of cytokinin, fusicoccin, and ethylene on auxin-induced changes in gene expression during auxin-promoted cell elongation in soybean (Glycine max L. Merr. cv Wayne) using cloned cDNAs to two auxin-responsive mRNAs (Walker, Key 1982 Proc Natl Acad Sci USA 79: 7185-7989). RNA blot analyses demonstrate that under conditions of cytokinin inhibition of auxin-promoted cell elongation the levels of these two auxin-responsive mRNAs is unaltered. Fusicoccin-promoted elongation is not associated with an enhanced expression of these two mRNAs, suggesting that the increased levels of these mRNAs observed during auxin-promoted cell elongation are not simply due to enhanced rates of cell elongation. We have also determined that ethylene plays no apparent role in the regulation of expression of these mRNAs. However, the auxins indole-3-acetic acid, 2,4-dichlorophenoxyacetic acid, and {alpha}-naphthalene acetic acid all enhance an accumulation of these mRNAs. We conclude that the regulation of these mRNAs is directly dependent on auxin. That auxin-promoted cell elongation is dependent upon the increased accumulation of these mRNAs remains to be determined.

2 Present address: Institute of Biology, Department of Plant Physiology, Adam MicKiewicz University, 61-713 Poznan, Poland.

1 Supported by National Institutes of Health Grant GM 30317 to J. L. K.






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ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1985 by the American Society of Plant Biologists