Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
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Plant Physiology 77:926-929 (1985)
© 1985 American Society of Plant Biologists

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Articles

Determination of Endogenous Indole-3-Acetic Acid in Plagiochila arctica (Hepaticae) 1

David M. Law, Dominick V. Basile and Margaret R. Basile

Department of Biology, The Pennsylvania State University, University Park, Pennsylvania 16802, Department of Biological Sciences, H. H. Lehman College, CUNY, Bronx, New York 10468

Endogenous indole-3-acetic acid (IAA) was found in axenically cultured gametophytes of the leafy liverwort, Plagiochila arctica Bryhn and Kaal., by high-performance liquid chromatography with electrochemical detection. Identification of the methylated auxin was confirmed by gas chromatography-mass spectrometry. Addition of 57 micromolar IAA to cultures increased relative production of ethylene. This is the first definitive (gas chromatography-mass spectrometry) demonstration of the natural occurrence of IAA in a bryophyte.

1 Supported in part by Faculty Research Award No. 14054 from the Research Foundation of The City University of New York, and is published as contribution No. 231 of the Department of Biology, The Pennsylvania State University.




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A. Ester Sztein, J. D. Cohen, I. G. de la Fuente, and T. J. Cooke
Auxin metabolism in mosses and liverworts
Am. J. Botany, November 1, 1999; 86(11): 1544 - 1555.
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