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Plant Physiology 77:952-955 (1985)
© 1985 American Society of Plant Biologists

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Articles

Identification and Quantification of Indole-3-methanol in Etiolated Seedlings of Scots Pine (Pinus sylvestris L.) 1

Björn Sundberg, Göran Sandberg and Einar Jensen

Department of Forest Genetics and Plant Physiology, The Swedish University of Agricultural Sciences, S-901 83 Umeå, Sweden, Institute of Biology and Geology, University of Tromsø, N-9001 Tromsø, Norway

Combined gas chromatography-mass spectrometry has been used to identify indole-3-methanol in a purified buffer extract from etiolated seedlings of Pinus sylvestris L. Quantitative estimates obtained by high performance liquid chromatography with fluorescence detection, corrected for losses occurring during purification, indicated that etiolated seedlings of P. sylvestris contained 19.7 ± 1.4 nanograms (± standard deviation) indole-3-methanol per gram fresh weight. The stability of indole-3-methanol at different pH levels was investigated. The rate of conversion, to a less polar unidentified substance, was enhanced with increasing acidity.

1 Financial support was provided by the Swedish Council for Forestry and Agricultural Research.






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Copyright © 1985 by the American Society of Plant Biologists