Plant Physiol. Tips for Better Browsing
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Plant Physiology 78:139-143 (1985)
© 1985 American Society of Plant Biologists

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (16)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Philosoph-Hadas, S.
Right arrow Articles by Aharoni, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Philosoph-Hadas, S.
Right arrow Articles by Aharoni, N.
Agricola
Right arrow Articles by Philosoph-Hadas, S.
Right arrow Articles by Aharoni, N.
Articles

Carbohydrates Stimulate Ethylene Production in Tobacco Leaf Discs 1

II. Sites of Stimulation in the Ethylene Biosynthesis Pathway

Sonia Philosoph-Hadas, Shimon Meir and Nehemia Aharoni

Department of Fruit and Vegetable Storage, Agricultural Research Organization, The Volcani Center, Bet Dagan 50250, Israel

Galactose, sucrose, and glucose (50 millimolar) applied to tobacco leaf discs (Nicotiana tabacum L. cv `Xanthi') during a prolonged incubation (5-6 d) markedly stimulated ethylene production which, in turn, could be inhibited by aminoethoxyvinylglycine (2-amino-4-(2'-aminoethoxy)-trans-3-butenoic acid) (AVG) or Co2+ ions. These three tested sugars also stimulated the conversion of L-[3,4-14C]methionine to [14C]1-amino-cyclopropane-1-carboxylic acid (ACC) and to [14C]ethylene, thus indicating that the carbohydrates-stimulated ethylene production proceeds from methionine via the ACC pathway. Sucrose concentrations above 25 mM considerably enhanced ACC-dependent ethylene production, and this enhancement was related to the increased respiratory carbon dioxide. However, sucrose by itself could directly promote the step of ACC conversion to ethylene, since low sucrose concentrations (1-25 mM) enhanced ACC-dependent ethylene production also in the presence of 15% CO2.

The data suggest that the stimulation of ethylene production by sugars in tobacco leaf discs results from enhancement of ACC formation as well as from the conversion of ACC to ethylene, when both steps could be involved in regulation of ethylene biosynthesis.

1 Supported by grant No. I-145-79 from BARD, The United States-Israel Binational Agricultural Research and Development Fund. Contribution from the Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel. No. 1048-E, 1984 series.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1985 by the American Society of Plant Biologists