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Plant Physiology 78:178-182 (1985)
© 1985 American Society of Plant Biologists

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Analysis of Leaf Proteins by Two-Dimensional Gel Electrophoresis

Protease Action as Exemplified by Ribulose Bisphosphate Carboxylase/ Oxygenase Degradation and Procedure to Avoid Proteolysis during Extraction

Catherine Colas des Francs, Hervé Thiellement and Dominique de Vienne

Laboratoire de Génétique des Systèmes Végétaux, G.I.S. Moulon, 91190 Gif-sur-Yvette, France

A previous genetic analysis by two-dimensional electrophoresis of wheat leaf proteins led us to hypothesize that several polypeptides were degradation products of the large subunit of ribulose bisphosphate carboxylase/oxygenase (EC 4.1.1.39).

Various extraction procedures including the use of proteinase inhibitors allowed us to: (a) confirm that the suspected polypeptides are indeed degradation products; (b) find out under which conditions the proteinases act; (c) find a method which prevents degradation, by boiling the extract in the presence of sodium dodecyl sulfate and 2-mercaptoethanol.




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M. D. Johnson and X. Wang
Differentially Expressed Forms of 1-L-myo-Inositol-1-Phosphate Synthase (EC) in Phaseolus vulgaris
J. Biol. Chem., July 19, 1996; 271(29): 17215 - 17218.
[Abstract] [Full Text] [PDF]


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Copyright © 1985 by the American Society of Plant Biologists