Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Plant Physiology 78:405-410 (1985)
© 1985 American Society of Plant Biologists

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (26)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Neuburger, M.
Right arrow Articles by Douce, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Neuburger, M.
Right arrow Articles by Douce, R.
Agricola
Right arrow Articles by Neuburger, M.
Right arrow Articles by Douce, R.
Articles

Transport of NAD+ in Percoll-Purified Potato Tuber Mitochondria

Inhibition of NAD+ Influx and Efflux by N-4-Azido-2-nitrophenyl-4-aminobutyryl-3'-NAD+

Michel Neuburger, David A. Day1 and Roland Douce

Physiologic Cellulaire Végétale (U.A.-C.N.R.S. N. 576) DRF/BV, Centre d'Études Nucleaires et Universite Scientifique et Medicale, 85X F3804 Grenoble Cedex, France

A mechanism by which intact potato (Solanum tuberosum) mitochondria may regulate the matrix NAD content was studied in vitro. If mitochondria were incubated with NAD+ at 25°C in 0.3 molar mannitol, 10 millimolar phosphate buffer (pH 7.4), 5 millimolar MgCl2, and 5 millimolar {alpha}-ketoglutarate, the NAD pool size increased with time. In the presence of uncouplers, net uptake was not only inhibited, but NAD+ efflux was observed instead. Furthermore, the rate of NAD+ accumulation in the matrix space was strongly inhibited by the analog N-4-azido-2-nitrophenyl-4-aminobutyryl-3'-NAD+. When suspended in a medium that avoided rupture of the outer membrane, intact purified mitochondria progressively lost their NAD+ content. This led to a slow decrease of NAD+-linked substrates oxidation by isolated mitochondria The rate of NAD+ efflux from the matrix space was strongly temperature dependent and was inhibited by the analog inhibitor of NAD+ transport indicating that a carrier was required for net flux in either direction. It is proposed that uptake and efflux operate to regulate the total matrix NAD pool size.

1 Present address: Botany Department, The Australian National University, G.P.O. Box 4, Canberra, A.C.T. 2601 Australia.




This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
F. Palmieri, B. Rieder, A. Ventrella, E. Blanco, P. T. Do, A. Nunes-Nesi, A. U. Trauth, G. Fiermonte, J. Tjaden, G. Agrimi, et al.
Molecular Identification and Functional Characterization of Arabidopsis thaliana Mitochondrial and Chloroplastic NAD+ Carrier Proteins
J. Biol. Chem., November 6, 2009; 284(45): 31249 - 31259.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1985 by the American Society of Plant Biologists