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Plant Physiology 78:411-416 (1985)
© 1985 American Society of Plant Biologists

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Articles

Selection of Glyphosate-Tolerant Tobacco Calli and the Expression of this Tolerance in Regenerated Plants 1

Susan R. Singer and Carl N. McDaniel

Department of Biology, Rensselaer Polytechnic Institute, Troy, New York 12180

From nonmutagenized haploid suspensions of Nicotiana tabacum L. cv Wisconsin 38 cells, 51 cell lines capable of growth in the presence of 1 millimolar glyphosate (N-phosphonomethyl glycine) were initially isolated at a frequency of 2.3 x 10–8. Eighteen cell lines retained tolerance when grown on selective medium for 3 years. Tolerance persisted for at least 14 months in six cell lines cultured in the absence of glyphosate. Some plants regenerated from four glyphosate-tolerant cell lines were tolerant. Glyphosate-tolerant tissue was isolated from some sensitive as well as some tolerant regenerated plants. Six of the tolerant cell lines were also tolerant to the herbicide amitrole (3-amino-1,2,4-triazole). Five cell lines selected for amitrole tolerance were glyphosate tolerant. Some plants regenerated from three of these five cell lines were glyphosate tolerant and glyphosate-tolerant tissue was obtained from several of these regenerated plants. Amitrole uptake in suspension cultures of several variants was assessed in terms of influx rate constants. This parameter was not sufficiently different indicating that altered membrane properties could not account for the herbicide tolerance.

1 Supported in part by a grant from the United States Department of Agriculture, Science and Education administration, under Grant No. 5901-0410-8-0075-0 from the Competitive Research Grants Office, to C.N.M. and a National Science Foundation Graduate Fellowship to S.R.S.






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Copyright © 1985 by the American Society of Plant Biologists