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Plant Physiology 78:417-419 (1985)
© 1985 American Society of Plant Biologists

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Articles

Identification of Gibberellins A1, A5, A29, and A32 from Immature Seeds of Apricot (Prunus armeniaca L.) 1

Ruben Bottini2, Guillermina de Bottini2, Masaji Koshioka3, Richard P. Pharis and Bryan G. Coombe

Plant Physiology Research Group, Department of Biology, The University of Calgary, Calgary, Alberta, Canada T2N 1N4, Department of Plant Physiology, Waite Agricultural Research Institute, The University of Adelaide, Glen Osmond, South Australia 5064, Australia

Gibberellins (GAs) A1, A5, and A29 were identified, and also GA32 was confirmed, as endogenous GAs of immature seeds (3-4 weeks after anthesis, 0.25-0.5 gram fresh weight) of apricot (Prunus armeniaca L.) based on capillary gas chromatography (GC), retention time (Rt), and selected ion monitoring (SIM), in comparison with authentic standards. Fractions subjected to GC-SIM were purified and separated using sequential solvent partitioning -> paper chromatography -> reverse phase C18 high performance liquid chromatography (HPLC) -> bioassay on dwarf rice cv Tan-ginbozu. Two other peaks of free GA-like bioactivity (microdrop and immersion dwarf rice assays) were eluted from C18 HPLC at Rts where GA4/7 and GA8 (or other GAs with similar structures) would elute. Also, three unidentified GA glucoside-like compounds (based on bioactivity on the immersion assay, and no bioactivity on the microdrop assay) were noted. There were very high amounts of GA32 (112 ng of GA3 equivalents per gram fresh weight), and minor amounts (0.5 ng of GA3 equivalents) for each of GA1 and GA5, respectively, based on the microdrop assay.

2 Present address: Departamento de Ciencias Naturales, Universidad Nacional de Rio Cuarto, 5800 Rio Cuarto, Argentina.

3 Present address: National Institute of Agro-Environmental Sciences, Division of Pesticides, Kannondai 3-1-1, Yatabe-Machi, Tsukuba-gun, Ibaraki-ken 305, Japan.

1 Supported by Natural Sciences and Engineering Research Council of Canada, Grant A-2585, and International Scientific Exchange Award IS-0084 to R.P.P.






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Copyright © 1985 by the American Society of Plant Biologists