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Plant Physiology 78:459-465 (1985)
© 1985 American Society of Plant Biologists

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Final Step of Phosphatidic Acid Synthesis in Pea Chloroplasts Occurs in the Inner Envelope Membrane 1

Jaen Andrews2, John B. Ohlrogge and Kenneth Keegstra

Botany Department, University of Wisconsin, Madison, Wisconsin 53706, USDA-Northern Regional Research Center, 1815 North University Street, Peoria, Illinois 61604

The second enzyme of phosphatidic acid synthesis from glycerol-3-phosphate, 1-acylglycerophospate acyltransferase, was localized to the inner envelope membrane of pea chloroplasts. The activity of this enzyme was measured by both a coupled enzyme assay and a direct enzyme assay. Using the coupled enzyme assay, phosphatidic acid phosphatase was also localized to the inner envelope membrane, although this enzyme has very low activity in pea chloroplasts. The addition of UDP-galactose to unfractionated pea chloroplast envelope preparations did not result in significant conversion of newly synthesized diacylglycerol to monogalactosyldiacylglycerol. Thus, the envelope synthesized phosphatidic acid may not be involved in galactolipid synthesis in pea chloroplasts.

2 Supported by National Institutes of Health Grant 5 T32 GM07215. Current address: ARCO Plant Cell Research Institute, 6560 Trinity Court, Dublin, CA 94568.

1 Supported in part by a grant from the United States Department of Agriculture Competitive Research Grants Office




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 J. Biol. Chem.Home page
Y. Nakamura, M. Tsuchiya, and H. Ohta
Plastidic Phosphatidic Acid Phosphatases Identified in a Distinct Subfamily of Lipid Phosphate Phosphatases with Prokaryotic Origin
J. Biol. Chem., September 28, 2007; 282(39): 29013 - 29021.
[Abstract] [Full Text] [PDF]


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Copyright © 1985 by the American Society of Plant Biologists