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Target Molecular Size of the Red Beet Plasma Membrane ATPase ¹

Plant Biochemistry and Bioregulation Laboratory, United States Department of Agriculture-Agricultural Research Service, Utah State University, UMC 63, Logan, Utah, 84322, Department of Experimental Oncology, University of Utah Medical Center, Salt Lake City, Utah 84112

Radiation inactivation of the red beet (Beta vulgaris L.) plasma membrane ATPase was carried out using -ray radiation from a ¹³⁷Cs source. Inactivation of vanadate-sensitive ATPase activity by -ray radiation followed an exponential decline with increasing total dose, indicating a single target size calculated to have a molecular weight of about 228,000. Since the catalytic subunit of the red beet plasma membrane ATPase has been demonstrated to have a molecular weight of about 100,000 by dodecyl-sulfate gel electrophoresis following ³²P-phosphorylation, it is suggested that the native enzyme may exist, at least, as a dimer of catalytic subunits.